Last month, First Minister Nicola Sturgeon accused pro-Brexit ministers in London of 'grabbing power' from Scotland by refusing to honour referendum pledges to protect the country's farmers.
The Prime Minister issued a rallying call for the union insisting the "case has never been stronger" during an address to delegates at the Scottish Conservative conference in Glasgow today.
But she warned that the Scottish National Party (SNP), which dominates the Scottish parliament, was failing on education and on raising living standards.
The SNP, Scotland's main independence party, remains bullish on the subject, with MP Angus MacNeil telling Julia Hartley-Brewer that Theresa May knows another referendum would result in a secessionist victory.
And she will slam the SNP's performance on education, blaming failings on their "obsession" with a United Kingdom split.
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Britain's Prime Minister Theresa May, seeking to head off renewed calls for Scottish independence, is set Friday to warn Scots that leaving the United Kingdom would put their living standards at risk. I think what people want to see is the SNP government looking at the issues that matter to them on a day-to-day basis: the Scottish economy, reform of schools, jobs, the NHS.
Her speech, using some of the toughest language on Scotland since she became prime minister after Britain voted to leave the European Union last June, was a warning sign to Scotland that she was determined to keep the United Kingdom together under her watch. "I think the arguments are weaker and I think the people of Scotland are just as switched on as they were three years ago so I think there's every chance that we would win by a wider margin". "The SNP Government demands further powers for the Scottish Parliament, but fails to pass powers on to local people in Scotland's villages, towns and cities".
Ms Sturgeon hit back, accusing the Mrs May of using "the language of Westminster diktat" and claiming her ministers have sought "consensus and cooperation".
"Where we have been prepared to offer a solution short of our ideal outcome, they have refused to seriously engage". It was described by the SNP as a once-in-a-generation vote that took place at that time. In a speech to the Scottish Tory conference in Glasgow, May attempted to turn the tables on Sturgeon.
Michael Russell, Scotland's Brexit minister, said on Twitter: "Most glaring example of "tunnel vision" in United Kingdom today is Tory (Conservative) obsession with immigration which is driving disastrous Brexit process".